Rapid susceptibility testing for herpes simplex virus type 1 using real-time PCR

Analysis of Herpes Simplex Virus in Suspected Encephalitis, Keratitis and Dermal Infections Using Real- Time PCR

Background & Objectives: Herpes viruses can cause diseases in the clinical range. The virus can cause infection in various body parts, especially eyes and nervous system. The aim of this study was at evaluating  the Real-Time TaqMan probe PCR in diagnosing and monitoring of the patients with suspected HSV infections.Materials & Methods: More than a thousand patients with suspected HSV infection...

Loop-Mediated Isothermal Amplification (LAMP) for the Rapid Diagnosis of Herpes Simplex Virus Type 1 (HSV-1)

Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...

Real-Time PCR and Varicella-Zoster Virus Infections by Rapid Detection of Herpes Simplex Virus

Application of real-time PCR for determination of antiviral drug susceptibility of herpes simplex virus.

A quantitative real-time PCR (TaqMan) assay was developed for determination of antiviral drug susceptibility of herpes simplex virus (HSV). After short-time culture of the virus, the antiviral drug susceptibility of HSV isolates for acyclovir (ACV) was determined by measuring the reduction of the HSV type 1 (HSV-1) DNA levels in culture supernatants using real-time PCR. The 50% inhibitory conce...

Rapid detection of herpes simplex virus and varicella-zoster virus infections by real-time PCR.

The herpes simplex viruses types 1 and 2 (HSV-1 and HSV-2) and varicella-zoster virus (VZV) can cause life-threatening infections of the central nervous system and lead to severe infections in immunocompromised subjects and newborns. In these cases, rapid diagnosis is crucial. We developed three different real-time PCR assays based on TaqMan chemistry for the LightCycler instrument to detect HS...